Analysis of GAD65 Autoantibodies in Stiff-Person Syndrome Patients
نویسندگان
چکیده
منابع مشابه
Analysis of GAD65 autoantibodies in Stiff-Person syndrome patients.
Autoantibodies to the 65-kDa isoform of glutamate decarboxylase GAD65 (GAD65Ab) are strong candidates for a pathological role in Stiff-Person syndrome (SPS). We have analyzed the binding specificity of the GAD65Ab in serum and cerebrospinal fluid (CSF) of 12 patients with SPS by competitive displacement studies with GAD65-specific rFab-derived from a number of human and mouse mAbs specific for ...
متن کاملHigh definition profiling of autoantibodies to glutamic acid decarboxylases GAD65/GAD67 in stiff-person syndrome.
Highly reliable biomarkers for the diagnosis of neurological diseases are not widely available. Here we evaluated a luciferase immunoprecipitation technology (LIPS) for the diagnosis of a CNS autoimmune disorder, stiff-person syndrome (SPS). Analysis by LIPS of 40 sera samples from SPS and control subjects for anti-GAD65 antibodies revealed dramatic titer differences allowing diagnosis of SPS w...
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Glutamic acid decarboxylase 65 (GAD65) and autoantibodies specific for GAD65 (GADA) are associated with autoimmune diseases including Stiff Person Syndrome (SPS) and Type 1 diabetes (T1D). GADA is recognized as a biomarker of value for clinical diagnosis and prognostication in these diseases. Nonetheless, it remains medically interesting to develop sensitive and specific assays to detect GAD65 ...
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A 37-year-old man was admitted to our hospital in 1998 with severe contraction of both masseter muscles, which made him unable to open his mouth. He could only drink using a straw fitting through a tooth gap. Spasms of the facial and masseter muscles had started in 1993 and transiently improved after treatment with benzodiazepines. The patient’s history included treatment of back and joint pain...
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Stiff person syndrome (SPS) is a rare, neurological disorder characterized by sudden cramps and spasms. High titers of enzyme-inhibiting IgG autoantibodies against the 65 kD isoform of glutamic acid decarboxylase (GAD65) are a hallmark of SPS, implicating an autoimmune component in the pathology of the syndrome. Studying the B cell compartment and the anti-GAD65 B cell response in two monozygot...
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ژورنال
عنوان ژورنال: The Journal of Immunology
سال: 2005
ISSN: 0022-1767,1550-6606
DOI: 10.4049/jimmunol.175.11.7755